Science behind Daily Capsule
Most magnesium supplements end at the mineral. Let Loose doesn't.
We start with magnesium oxide — an osmotic mineral that draws water into the colon to soften stool and support regularity — and then we load it with active oxygen through a proprietary ozonation process. The result is a capsule with two jobs: it helps move what's stuck, and it supports the gut environment around it.
Active oxygen content measured by iodometric titration, manufacturer lab testing, 2026.
Tested in the lab. 13× the active oxygen.
Active oxygen is what makes an "ozonated" magnesium actually ozonated. We submitted Let Loose and the two most recognized alternatives to our manufacturer's laboratory and ran iodometric titration — the standard analytical method for quantifying active oxygen content in a dosed supplement.
Here's what came back:
26× more than Mg Oxide
In plain English: the magnesium does the physical work — pulling water into the colon to soften stool. The active oxygen does the supporting work — helping maintain a healthy gut environment. More oxygen per dose means more of that support in every capsule.
What ozonated magnesium oxide actually is
Ozonation is a process that binds oxygen (O₃) to another molecule, stabilizing it so it can travel somewhere oxygen doesn't usually reach. Our manufacturer pressurizes medical-grade ozone into magnesium oxide, producing a stable magnesium peroxide complex. Once the capsule dissolves, that complex begins releasing active oxygen into the digestive tract alongside the magnesium itself.
What happens when you take a Let Loose capsule:
Water moves in.
Magnesium oxide is an osmotic — it pulls water across the intestinal wall and into the colon. That hydrates stool, softens anything that's been sitting too long, and makes it easier to pass. This is the exact mechanism studied in randomized controlled trials of magnesium oxide for occasional constipation.1
Oxygen gets released.
As the magnesium peroxide complex dissociates in the digestive tract, it releases active oxygen into the gut environment — a region of the body where free oxygen is normally very scarce.
The real superpower isn't the magnesium. It's the oxygen.
Most of the constipation category talks about magnesium. Almost no one talks about what oxygen is doing in your gut. Here's what the peer-reviewed research shows.
Oxygen helps support a balanced gut environment.
Dysbiosis — an imbalance of gut bacteria often behind bloating and sluggish digestion — is driven in part by the overgrowth of anaerobic species that thrive in a low-oxygen, stagnant colon. In a 2025 peer-reviewed study published in the Journal of Medical Microbiology, ozonated water administration in a dysbiosis model increased beneficial microbiota populations and supported intestinal barrier function, working through the SIRT1–Nrf2/HO-1 signaling pathway.3
Plain English: oxygen in the gut helps tilt the balance back toward the bacteria you want.Oxygen supports the gut barrier.
The gut barrier is a single-cell-thick layer held together by "tight junction" proteins. When it's doing its job, it lets nutrients through and keeps everything else out. Preclinical research shows that ozone/oxygen therapy supports tight-junction integrity: in one 2025 study, it helped preserve barrier function by activating the aryl hydrocarbon receptor and modulating tryptophan metabolism.4 A separate preclinical model found that medical ozone supported the body's endogenous antioxidant defenses in intestinal tissue.5
Plain English: oxygen helps the gut lining stay sealed the way it's supposed to.Oxygen creates an environment less hospitable to unwanted microbes.
Ozone's mechanism of action on bacteria is well characterized. It oxidizes microbial cell membranes, disrupts sulfhydryl groups, and damages pathogen DNA.67 The species most sensitive to this pressure are strict anaerobes — exactly the population that tends to overgrow in a compacted, sluggish colon.
Plain English: the microbes that cause the most trouble in a stagnant gut are the ones that hate oxygen the most.Oxygen supports the body's natural antioxidant defenses.
Counterintuitive but well-documented: therapeutic ozone/oxygen mixtures have been shown to increase the body's endogenous antioxidant capacity — upregulating the Nrf2 pathway, glutathione, and superoxide dismutase — rather than deplete it.8
Plain English: a small, controlled dose of active oxygen prompts the body to build more of its own defense system, not less.Magnesium oxide beats placebo. Ours starts there.
We're keeping the existing RCT evidence on this page because it's the strongest human data for the mineral itself. The reframe: this is the baseline — what magnesium oxide does on its own, before we add active oxygen.
In a 2019 randomized, double-blind, placebo-controlled trial published in the Journal of Neurogastroenterology and Motility, magnesium oxide improved overall symptoms in 70.6% of participants with occasional constipation, compared with 25% on placebo.1 A 2020 follow-up RCT confirmed it: magnesium oxide matched the efficacy of senna (a stimulant laxative) at roughly 68% response, with a different, non-stimulant mechanism.9
Now imagine that baseline — plus 28,186 ppm of active oxygen in every dose.
(2019 RCT)
(same trial)
non-stimulant mechanism (2020 RCT)
One capsule. Two mechanisms. Non-habit-forming.
Let Loose is the only daily capsule we know of that pairs an RCT-studied osmotic mineral with lab-verified active oxygen at 13× the nearest competitor. The magnesium helps move things along. The oxygen supports the environment it's moving through. You take it. You wake up lighter.
These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. If you are pregnant, nursing, taking medication, or have a medical condition, consult your healthcare provider before use.
References
- Mori S, et al. (2019). A Randomized Double-blind Placebo-controlled Trial on the Effect of Magnesium Oxide in Patients With Chronic Constipation. J Neurogastroenterol Motil.
- Fontes B, et al. (2012). Effect of low-dose gaseous ozone on pathogenic bacteria. BMC Infectious Diseases.
- Ozone water enema activates SIRT1-Nrf2/HO-1 pathway to ameliorate gut dysbiosis (2025). Journal of Medical Microbiology.
- Ozone controls the metabolism of tryptophan protecting against sepsis-induced intestinal damage by activating aryl hydrocarbon receptor (2025). World Journal of Gastroenterology.
- Guven A, et al. (2009). Medical ozone therapy reduces oxidative stress and intestinal damage in an experimental model of necrotizing enterocolitis in neonatal rats. Journal of Pediatric Surgery.
- Cho M, et al. (2011). Assessing the microbial oxidative stress mechanism of ozone treatment through the responses of Escherichia coli mutants. Journal of Applied Microbiology.
- Epelle EI, et al. (2023). Ozone application in the food industry: mechanisms of action on microorganisms. PMC.
- Bocci V, et al. (2010). Effect of ozone/oxygen mixture on systemic oxidative stress and organic damage. Toxicology and Industrial Health.
- Morishita D, et al. (2020). Senna Versus Magnesium Oxide for the Treatment of Chronic Constipation: A Randomized, Placebo-Controlled Trial. American Journal of Gastroenterology.