Science behind Daily Capsule
Most magnesium supplements end at the mineral. Let Loose doesn't.
We start with magnesium oxide — an osmotic mineral that draws water into the colon to soften stool and support regularity — and then put it through our proprietary ozonation process, creating a highly ozonated form of magnesium with measurable active oxygen. The result is a formula designed to support gentle, effective elimination from more than one angle.
Active oxygen content measured by iodometric titration, manufacturer lab testing, 2026.
Tested in the lab. 13× the active oxygen.
Active oxygen is what makes an "ozonated" magnesium actually ozonated. We submitted Let Loose and the two most recognized alternatives to our manufacturer's laboratory and ran iodometric titration — the standard analytical method for quantifying active oxygen content in a dosed supplement.
Here's what came back:
In plain English: magnesium oxide does the proven osmotic work — drawing water into the colon to soften stool and support regularity. What makes Let Loose different is our proprietary ozonation process, which creates a highly ozonated form of magnesium with 13× more measurable active oxygen than the leading ozonated competitor tested.
What ozonated magnesium oxide actually is
Ozonation is a proprietary process that incorporates oxygen into our magnesium formula using medical-grade ozone. The result is our unique ozonated magnesium ingredient, developed to support digestive health and regularity. That’s what makes Let Loose different.*
What happens when you take a Let Loose capsule:
Water moves in.
Magnesium oxide draws water into the colon to help soften stool and support regular bowel movements.1
Our proprietary ozonation technology.
Our unique manufacturing process enhances magnesium oxide using medical-grade ozone to create our patented ozonated magnesium ingredient.
The real superpower isn’t the magnesium. It’s our proprietary ozonation technology.
Most of the constipation category talks about magnesium. We wanted to explore whether our proprietary ozonation process could take it one step further. Here’s some of the peer-reviewed research that inspired our technology.
The studies below include laboratory and preclinical research on oxygen- and ozone-based approaches. They are provided as scientific background for our proprietary ozonation technology and are not clinical studies of Let Loose capsules.
Research is exploring how ozone may influence the gut environment.
Ozone is a highly reactive form of oxygen that researchers are studying for its effects on the intestinal environment. In a 2025 peer-reviewed study published in the Journal of Medical Microbiology, ozonated water administration in a preclinical dysbiosis model altered microbial populations and supported markers of intestinal barrier function through the SIRT1–Nrf2/HO-1 signaling pathway.3
Plain English: Researchers are studying how ozone-based approaches may influence the gut environment and microbiome. More research is needed to understand how these findings translate to oral ozonated magnesium.Research is exploring how ozone may support gut barrier function.
The gut barrier is a single-cell-thick layer held together by “tight junction” proteins. When functioning normally, it helps regulate what passes from your digestive tract into your body. Preclinical research has investigated how ozone-based approaches may interact with pathways involved in intestinal barrier integrity. In one 2025 study, researchers observed preservation of barrier function through activation of the aryl hydrocarbon receptor and modulation of tryptophan metabolism.4 A separate preclinical model found that medical ozone influenced the body's endogenous antioxidant defenses in intestinal tissue.5
Plain English: Researchers are continuing to explore how ozone-based approaches may support the intestinal barrier.Research suggests ozone can interact with microorganisms.
Ozone's interactions with microorganisms have been well characterized in laboratory research. Studies show that ozone can interact with microbial cell membranes and other cellular structures through oxidative mechanisms.67 Researchers are continuing to investigate how these properties may translate to applications involving the digestive environment.
Plain English: Ozone has well-documented interactions with microorganisms in laboratory settings. What that means for oral ozonated magnesium in humans still needs to be studied.Researchers are exploring ozone's relationship with the body's antioxidant response.
Preclinical and clinical research has investigated how controlled ozone-based therapies may influence the body's endogenous antioxidant pathways, including Nrf2, glutathione, and superoxide dismutase.8 While these findings are interesting, additional research is needed to understand how they may apply to oral dietary supplements.
Plain English: Scientists are investigating how controlled ozone exposure may interact with the body's own antioxidant defense systems.Magnesium oxide beats placebo. Ours starts there.
We're keeping the existing RCT evidence on this page because it's the strongest human data for the mineral itself. The reframe: this is the baseline — what magnesium oxide does on its own, before we add active oxygen.
In a 2019 randomized, double-blind, placebo-controlled trial published in the Journal of Neurogastroenterology and Motility, magnesium oxide improved overall symptoms in 70.6% of participants with occasional constipation, compared with 25% on placebo.1 A 2020 follow-up RCT confirmed it: magnesium oxide matched the efficacy of senna (a stimulant laxative) at roughly 68% response, with a different, non-stimulant mechanism.9
Now imagine that baseline — plus 28,186 ppm of active oxygen in every dose.
(2019 RCT)
(same trial)
non-stimulant mechanism (2020 RCT)
One capsule. Magnesium + proprietary ozonation. Non-habit-forming.
Let Loose pairs an RCT-studied osmotic mineral with our proprietary ozonation technology and 13× more measurable active oxygen than the leading ozonated competitor tested. Magnesium oxide helps draw water into the colon to soften stool and support regularity. Our ozonation process is what makes Let Loose different from ordinary magnesium oxide—and we're continuing to study the additional role this unique chemistry may play in digestive health.*
These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. If you are pregnant, nursing, taking medication, or have a medical condition, consult your healthcare provider before use.
References
- Mori S, et al. (2019). A Randomized Double-blind Placebo-controlled Trial on the Effect of Magnesium Oxide in Patients With Chronic Constipation. J Neurogastroenterol Motil.
- Fontes B, et al. (2012). Effect of low-dose gaseous ozone on pathogenic bacteria. BMC Infectious Diseases.
- Ozone water enema activates SIRT1-Nrf2/HO-1 pathway to ameliorate gut dysbiosis (2025). Journal of Medical Microbiology.
- Ozone controls the metabolism of tryptophan protecting against sepsis-induced intestinal damage by activating aryl hydrocarbon receptor (2025). World Journal of Gastroenterology.
- Guven A, et al. (2009). Medical ozone therapy reduces oxidative stress and intestinal damage in an experimental model of necrotizing enterocolitis in neonatal rats. Journal of Pediatric Surgery.
- Cho M, et al. (2011). Assessing the microbial oxidative stress mechanism of ozone treatment through the responses of Escherichia coli mutants. Journal of Applied Microbiology.
- Epelle EI, et al. (2023). Ozone application in the food industry: mechanisms of action on microorganisms. PMC.
- Bocci V, et al. (2010). Effect of ozone/oxygen mixture on systemic oxidative stress and organic damage. Toxicology and Industrial Health.
- Morishita D, et al. (2020). Senna Versus Magnesium Oxide for the Treatment of Chronic Constipation: A Randomized, Placebo-Controlled Trial. American Journal of Gastroenterology.