The Science Behind Ozonated Magnesium Oxide: Clinically Proven for Digestive Health
Backed by clinical research, Ozonated Magnesium Oxide is the breakthrough digestive solution that relieves constipation, detoxifies the gut, and supports long-term gut health—without harsh laxatives
Read the Full Study
Backed by Clinical Research & Designed for Better Gut Health
For centuries, magnesium compounds have been used to support digestion, with magnesium oxide (MgO) being one of the most effective. Research from the Nutrients Journal (PMC Study) confirms that magnesium oxide is a safe, well-tolerated laxative that supports bowel regularity, detoxification, and gut health—without harsh side effects.
Unlike traditional laxatives that cause dependency, magnesium oxide works by drawing water into the intestines, softening stool naturally. This means no cramping, no bloating, and no harsh urgency—just smooth, regular digestion.
What the Science Says
- Proven to Relieve Occasional Constipation – Studies show magnesium oxide enhances water retention in the gut, leading to softer stools and easier elimination.
- Detoxifies the Digestive System – Helps remove waste and toxins while supporting a healthy gut microbiome.
- Safe for Long-Term Use – Unlike stimulant laxatives, it doesn’t cause dependency or disrupt gut bacteria balance.
- Supports Overall Health – Linked to better hydration, muscle recovery, and improved sleep.
Ozonated Magnesium Oxide: A Next-Level Digestive Solution
How Let Loose Enhances the Power of Magnesium Oxide
Most magnesium oxide supplements are basic mineral compounds, but Let Loose uses an ozonated form for superior gut-cleansing effects. Ozonation is a specialized process that infuses magnesium oxide with oxygen to create a more bioavailable, highly effective digestive aid.
How Ozonated Magnesium Oxide Works
1
Increases Oxygen
Flow to the Gut
The ozonated formula helps oxidize and break down waste, promoting deep colon cleansing.
2
Supports Gentle,
Overnight Detox
Unlike stimulant laxatives, ozonated magnesium works without cramping or bloating.
3
Promotes Healthy
Bacteria Balance
By increasing oxygen in the gut, it helps support a thriving microbiome.
How Let Loose Uses This Research for Maximum Benefits
While most laxatives rely on stimulants that can disrupt digestion and cause urgency, Let Loose takes a different approach. Our oxygenated magnesium oxide formula is:
- ✔️ Doctor-Recommended – Backed by real science & GI experts.
- ✔️ Fast-Acting & Gentle – Works overnight without discomfort.
- ✔️ All-Natural, No Harsh Laxatives – No Senna, no dependency, just smooth relief.
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Daily Capsule For Smooth, Sress-Free Poops
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Experience the Science of Better Digestion
Let Loose is more than just another supplement—it’s backed by science, trusted by experts, and formulated for real digestive relief. No harsh chemicals. No dependency. Just better gut health, every day.
Final Thought: Backed by Research, Trusted by Experts
Dr. James H Tabibian, MD, PhD, Gastroenterologist, Let Loose Medical Advisor
“It’s uncomfortable, frustrating, and can disrupt a person’s lifestyle. That’s why I was excited to join the team behind Let Loose—a science-backed, natural supplement designed to tackle constipation head-on.”
– Dr. James H Tabibian, MD, PhD, Gastroenterologist
This scientific breakthrough in digestive health is transforming the way people experience gut relief. By merging clinical research with the power of oxygenated magnesium oxide, Let Loose delivers a modern solution to an age-old problem—without the side effects of harsh laxatives.
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The Science Behind Koji Digestive Enzymes
Digestion is the gateway to nutrition
You don't actually get nourished by what you eat. You get nourished by what you digest.
Modern meals are more complex than the digestive system they're landing in. Processed ingredients, dense proteins, refined oils, engineered foods — all of it asks more of your enzymes than nature ever planned for. Koji Digestive Enzymes help your body break food into the small, usable pieces it actually needs. Meals feel lighter. Digestion runs smoother. Your body gets more out of the food you're already eating.
355 mg
of functional enzymes per capsule.
4×
the total enzyme content of typical mass-market blends.
Most enzyme supplements are designed to sound powerful. Ours is dosed to actually perform.
Walk down the enzyme aisle and most labels look impressive: "18 enzymes!" "22-enzyme complex!" Flip them over. The total enzyme content is often around 85 mg, spread across a dozen or more ingredients in trace amounts. That's enough to list on a label. It's not always enough to do the work of digesting a real meal.
Here's the difference:
| Typical Mass-Market Blend | Let Loose Koji Digestive Enzymes |
|---|---|
| ~85 mg total blend | 355 mg per capsule |
| Dozens of enzymes listed, each in trace amounts | 16 enzymes dosed at activity levels sized for real meals |
| Often listed by weight only | Disclosed in enzyme activity units (HUT, DU, FIP, etc.) |
| Commodity sourcing across multiple suppliers | Traditional Japanese Koji fermentation, 150+ year process |
Why activity units matter:
An enzyme's weight tells you how much material is in the capsule, but that includes inactive components too. Activity units tell you how much digestive work it can actually perform. They're not the same thing. HUT (protease– breaks down protein), DU and SU (amylase- break down carbohydrates and starches), FIP (lipase- breaks down fat), GALU (lactase- breaks down lactose and dairy sugars), and ALU (alpha-galactosidase- helps break down beans and complex carbs). These are the standard units used to measure real enzyme function. We list every single one on our label.
What happens when you take a Let Loose capsule before a meal
You take it with water a few minutes before eating. The capsule dissolves in your stomach, releasing the Koji enzyme blend into the digestive tract right as food arrives. Each enzyme works on a specific part of the meal.
The three things that happen:
1. Protein gets a head start in the stomach.
Most animal-derived digestive enzymes don't survive stomach acid — they need enteric coating to work. Koji-derived enzymes are different: fungal proteases are naturally acid-stable and remain active under gastric conditions.1 Our formula includes acid-stable proteases that start breaking protein apart before it ever reaches the small intestine, loosening the structure so later enzymes can finish the job.2
2. The full meal gets covered.
Sixteen enzymes work in parallel — proteases on the steak, amylases on the bread, lipases on the oil, lactase on the cheese, cellulases on the vegetables, alpha-galactosidase on the beans. Whatever's on your plate, something in the capsule is working on it.
3. The good stuff becomes available.
Better breakdown means the nutrients locked inside plant cell walls, protein matrices, and fat droplets are released where your gut can actually absorb them. That's the whole point: you don't benefit from what you eat — you benefit from what you digest and absorb.
What's in each capsule:
See full enzyme breakdown
| Enzyme | Activity per capsule | What it breaks down |
|---|---|---|
| Protease I | 20,000 HUT | Proteins — meats, dairy, legumes |
| Protease II | 50,000 HUT | Proteins — broad pH range |
| Protease (Acid-Stable) | 200 SAPU | Starts protein breakdown in the stomach |
| Peptidase DPP-IV | 72 DPPIV | Peptides and gluten-derived fragments |
| Peptidase | 4,000 HUT | Shorter protein chains |
| Alpha Amylase | 12,000 DU | Starches — bread, rice, potato |
| Alpha Galactosidase | 300 GALU | Complex sugars in beans, cruciferous veg |
| Beta Glucanase | 50 BGU | Beta-glucans in oats, barley |
| Invertase | 400 SU | Sucrose |
| Lactase | 400 ALU | Lactose (dairy sugar) |
| Pectinase | 14 ENDO PG | Pectin in fruits and vegetables |
| Cellulase | 2,000 CU | Cellulose — plant cell walls |
| Hemicellulase | 2,000 HCU | Hemicellulose — plant fiber |
| Xylanase | 300 XU | Xylan — grain fiber |
| Lipase I | 100 FIP | Fats — short/medium-chain |
| Lipase II | 1,300 FIP | Fats — broad-chain |
Activity units: HUT = protein-breaking units. DU = starch-breaking units. FIP = fat-breaking units. GALU, SU, ALU, BGU, CU, HCU, XU, ENDO PG, DPPIV, SAPU = specialist units for specific substrates. Industry standard measurements.
What one capsule is sized for (theoretical)
Based on the activity levels above, one capsule is formulated to support digestion of roughly 40–60 g of protein, 40–80 g of carbohydrate, and 20–25 g of fat — about the size of a steak dinner with a baked potato. These estimates are biochemical modeling, not clinical claims; actual digestion varies by person.
Why we make this with a 1,100-year-old fermentation method
Most enzyme supplements on the market are made by isolating one or two enzymes from fungal or bacterial fermentation, then heavily processing and purifying them into a powder before blending them together. It's efficient. It's cheap. But it's also a narrow, reductionist approach to a very complex problem—because real food isn't made of isolated molecules, it's made of complex structures.
Koji (Aspergillus oryzae) is different. It's been cultivated in Japan for over a millennium — originally for miso, soy sauce, and sake — and was domesticated specifically for its enzyme production. A single Koji culture naturally secretes dozens of hydrolytic enzymes in parallel, including 65 endopeptidase and 69 exopeptidase genes' worth of protein-cutting machinery.3 It's the only fungus in the world with a dedicated status as a national fungus of Japan.
-
Koji is built for broad-spectrum digestion.
A single Koji fermentation produces a natural ecosystem of enzymes — proteases, amylases, lipases, cellulases, hemicellulases, xylanases, pectinases, peptidases, esterases — all at once. Because they evolved together, they function together. This is closer to how digestion actually works in the gut (coordinated, overlapping, redundant) than isolated single-enzyme blends can match.4
Plain English: Koji gives you a whole team, not a lineup of solo players.
-
Koji enzymes survive stomach acid.
In in-vitro models of human digestion, fungal enzymes remain active under gastric conditions — the high acidity that destroys animal-derived pancreatic enzymes (which is why pancreatic supplements require enteric coating).1 Our acid-stable proteases start breaking down protein in the stomach phase, before food even reaches the small intestine.
Plain English: they start working the moment you swallow.
-
Koji has 150+ years of modern medical use.
Koji-derived enzymes aren't new. In 1894, Japanese chemist Jokichi Takamine patented Taka-diastase — a Koji-derived digestive enzyme preparation — and it became one of the first enzyme supplements ever commercialized for stomach upset and overeating.5 Today, A. oryzae is GRAS-designated by the FDA for specified enzyme preparations.5
Plain English: this isn't a trend ingredient. Koji has been helping people digest meals since before the invention of the automobile.
-
Koji produces secondary enzymes that isolated blends miss.
Traditional Koji fermentation naturally generates a long tail of secondary enzymes beyond the headline list — cellulases, hemicellulases, xylanases, pectinases, esterases, and additional peptidases. These secondary enzymes specialize in breaking apart the complex structures that trap nutrients: plant cell walls, protein matrices, connective tissues, cellular membranes.
Plain English: before the main enzymes can do their job, something has to open the food up. Koji does both.
The takeaway: Koji fermentation is the reason this formula performs like an integrated digestive system instead of a list of isolated enzymes on a label.
What the research says about digestive enzyme supplementation
The category has become well-studied over the last decade. Here's what peer-reviewed human trials have shown.
Multi-enzyme supplementation reduces post-meal bloating.
A 2024 randomized, double-blind, placebo-controlled crossover study of 25 adults with daily post-meal bloating found that a single dose of a multi-digestive-enzyme supplement reduced abdominal distension by 58% at 30 minutes and 68% at 90 minutes compared to placebo. Eighty percent of participants saw a measurable reduction in distension with the enzyme product.6
Enzyme supplements improve digestive comfort over time.
A 2023 randomized, double-blind, placebo-controlled trial of 120 adults with digestive symptoms used a multi-enzyme supplement twice daily for two months. The enzyme group reported improvements in overall quality of life, sleep quality, and a decrease in the severity of post-meal discomfort — with no side effects reported.7 A separate RCT in functional digestive complaints likewise showed statistically significant improvements in bloating, fullness, and post-meal distress versus placebo.8
The underlying mechanism is now well-characterized.
A 2022 study using the INFOGEST in-vitro model of human digestion demonstrated that fungal-derived digestive enzymes meaningfully improve macronutrient hydrolysis — particularly for protein, starch, and fat — under simulated gastric and small intestinal conditions.1 In other words, the mechanism isn't speculative. You can watch it happen in the lab.
Our formula is built on the same class of enzymes these studies used — Koji-fermented, broad-spectrum, dosed by activity unit — at 355 mg per capsule.
One capsule before your two biggest meals. That's the whole ritual.
Take one Let Loose Koji Digestive Enzymes capsule before your two largest meals of the day. For heavier meals, take two. Small habit, meaningful support. And because Koji enzymes are naturally acid-stable, you don't have to time it perfectly — they start working as soon as the capsule dissolves.
Eat what you want. Just digest it better.
These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. If you are pregnant, nursing, taking medication, or have a medical condition, consult your healthcare provider before use. Discontinue if adverse reactions occur.
References
- Morell P, et al. (2022). Fungal digestive enzymes promote macronutrient hydrolysis in the INFOGEST static in vitro simulation of digestion. Food Chemistry.
- Enzyme Technical Association. Orally Administered Enzyme Food Supplement Safety Overview.
- Yang L, et al. (2022). Phenotypic, Genomic, and Transcriptomic Comparison of Industrial Aspergillus oryzae: Analysis of Key Proteolytic Enzymes Produced by Koji Molds. Microbiology Spectrum.
- Daba GM, et al. (2021). The ancient koji mold (Aspergillus oryzae) as a modern biotechnological tool. Bioresources and Bioprocessing.
- Hiroi M, Nagao S. (2021). Medical Application of Substances Derived from Non-Pathogenic Fungi Aspergillus oryzae and A. luchuensis-Containing Koji. Journal of Fungi (PMC).
- Spagnuolo R, et al. (2024). A Multi-Digestive Enzyme and Herbal Dietary Supplement Reduces Bloating in a Single Use in Healthy Adults: A Randomized, Placebo-Controlled, Cross Over Study. Nutrition and Dietary Supplements.
- Graziani C, et al. (2023). Efficacy of digestive enzyme supplementation in functional dyspepsia: A monocentric, randomized, double-blind, placebo-controlled, clinical trial. Biomedicine & Pharmacotherapy.
- Majeed M, et al. (2018). Evaluation of the Safety and Efficacy of a Multienzyme Complex in Patients with Functional Dyspepsia: A Randomized, Double-Blind, Placebo-Controlled Study. Journal of Medicinal Food.
How Let Loose TrioBiotic Works
Your gut is home to trillions of microbes and a delicate barrier that lines your intestines. When this ecosystem is out of balance, digestion may suffer - manifesting as irregularity, bloating, gas, or slow transit.
TrioBiotic supports gut health with a three-pronged approach:
- Probiotics – beneficial live microbes that survive digestion and reach the gut.
- Postbiotics – non-live microbial components that still trigger beneficial responses.
- Prebiotics & Resistant Starch – fibers that selectively feed good microbes and promote beneficial by-products (short-chain fatty acids) that nourish your gut lining and beyond.
Together, these ingredients promote:
- A stronger gut barrier (meaning fewer leaks & less inflammation)
- A healthier microbiome (more good bacteria, fewer “bad” ones)
- Better digestive regularity and comfort
- Support for metabolic health, immunity, and nutrient absorption
Bacillus coagulans (2 Billion CFU)
-
Mechanism highlights:
- Spore-forming resilience → survives stomach acid and bile to reach intestines. ScienceDirect+1
- Produces digestive enzymes and short-chain fatty acids → supports digestion and gut lining health. BioMed Central
- Modulates immune function and microbiota balance. ScienceDirect+1
-
Clinical benefits shown in human trials:
- Improves IBS symptoms: reduced abdominal pain & increased complete spontaneous bowel movements. Nature
- Safe at 2 × 10⁹ CFU/day in adults with diarrhea-predominant IBS. BioMed Central
-
Additional demonstrated benefits:
- Better stool regularity + improved stool consistency
- Reduction in occasional bloating and gas
- Enhanced nutrient absorption and digestive efficiency
- Support for immune and gut-brain axis pathways
Bacillus subtilis (2 Billion CFU)
-
Mechanism highlights:
- Spore-forming bacterium supporting a diverse microbiome and producing antimicrobial compounds inhibiting pathogens. Lippincott Journals
- Supports intestinal barrier integrity and healthy immune signalling.
-
Benefits for your site:
- Helps rebalance gut flora and crowd out less beneficial bacteria
- Strengthens gut lining to reduce “gut leakiness”
- May reduce occasional digestive inflammation, gas, & discomfort
- Supports immune resilience from the gut
Akkermansia muciniphila (Heat-Killed AH-39)
-
Mechanism highlights:
- Resides in the mucous layer of the gut, interacting with intestinal lining and influencing metabolism. [Gut+1]
- Even pasteurized (heat-killed) form shows metabolic and barrier benefits. [Nature+1]
-
Clinical insights:
- In overweight/insulin-resistant humans, supplementation correlated with improvements in insulin sensitivity, fat mass, and inflammation markers. [Nature+1]
-
Benefits for your site:
- Strengthens gut barrier & reduces intestinal permeability
- Supports healthy metabolism (weight, insulin, lipid balance)
- Promotes microbiome diversity and long-term digestive resilience
Xylooligosaccharides (XOS)
-
Mechanism highlights:
- Specific prebiotic fiber that selectively increases beneficial bacteria like Bifidobacteria. [PMC+1]
- Supports production of short-chain fatty acids and reduces gut pH → beneficial environment for gut health. [Cambridge University Press & Assessment]
-
Benefits for your site:
- Improves stool frequency & consistency (including relief from functional constipation)
- Supports smoother digestion & reduction in occasional bloating
- Helps maintain a resilient gut environment that supports metabolism and immunity
Galactooligosaccharides (GOS)
-
Mechanism highlights:
- Prebiotic fiber broadly supporting Bifidobacteria and Lactobacilli, especially in older adults. [Cambridge University Press & Assessment]
- Associated with improved mineral absorption and immune signalling.
-
Benefits for your site:
- Supports daily regularity and softer, more comfortable bowel movements
- Nourishes good bacteria and supports overall gut microbiome balance
- Helps support mineral absorption (calcium, magnesium) and immune health
Green Banana Powder (Resistant Starch)
-
Mechanism highlights:
- High in resistant starch (RS) which reaches the colon to fuel beneficial microbes and produce short-chain fatty acids (especially butyrate). [PMC+1]
- Supports gut barrier restoration and microbial balance. [PMC+1]
-
Benefits for your site:
- Promotes stool form, regularity, and digestive comfort
- Supports blood sugar balance and healthy metabolism
- Helps soothe and strengthen the intestinal lining
- Adds a natural prebiotic fiber that supports your microbiome’s long-term health
Hyperice
Advanced Daily TrioBiotic: Complete Pre + Pro + Postbiotic Support
$56
Summary
TrioBiotic from Let Loose is built on multiple layers of science:
- Two robust spore-forming probiotics (B. coagulans + B. subtilis) that survive the digestive tract and reach your intestines alive.
- A next-generation postbiotic (A. muciniphila heat-killed) with demonstrated benefits for gut lining & metabolism.
- Two potent prebiotic fibers (XOS + GOS) and a resistant starch source (green banana) to feed and empower your microbiome.
Together, this formula helps deliver smoother digestion, better regularity, a stronger gut barrier, a more resilient microbiome, and supportive metabolic & immune benefits – tailored for the young, active, health-conscious consumer.
Read the Papers
- Depommier C, et al. Supplementation with Akkermansia muciniphila in overweight/obese insulin-resistant volunteers: a randomized, double-blind, placebo-controlled pilot study. Nature Medicine 2019. [Nature]
- Madempudi R.S., et al. The effect of Bacillus coagulans Unique IS2 in adults with IBS: randomized double-blind study. Scientific Reports 2019. [Nature]
- Yan J., et al. Akkermansia muciniphila: is it the Holy Grail for ameliorating metabolic disorders? Gut Microbes 2021. [PMC]
- Yi W., et al. Xylo-oligosaccharides improve functional constipation by modulating the gut microbiota. Frontiers in Nutrition 2023. [PMC]
- Baek G.H., et al. Prebiotic potential of green banana flour: impact on gut microbiota composition in mice. Frontiers in Nutrition 2023. [Frontiers]
- Walton G.E., et al. A randomised crossover study investigating the effects of galacto-oligosaccharides on the faecal microbiota in men and women over 50 years of age. British Journal of Nutrition 2012. [Cambridge University Press & Assessment]
Legal/Scientific Disclaimer
These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. Individual results may vary. Please consult your healthcare provider before beginning any new supplement regimen, especially if you are pregnant, nursing, taking medications, or have a medical condition.