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Most magnesium supplements end at the mineral. Let Loose doesn't.
We start with magnesium oxide β an osmotic mineral that draws water into the colon to soften stool and support regularity β and then we load it with active oxygen through a proprietary ozonation process. The result is a capsule with two jobs: it helps move what's stuck, and it supports the gut environment around it.
28,186
ppm active oxygen per dose
13Γ
more than the leading competitor
26Γ
more than regular magnesium oxide
Active oxygen content measured by iodometric titration, manufacturer lab testing, 2026.
Tested in the lab. 13Γ the active oxygen.
Active oxygen is what makes an "ozonated" magnesium actually ozonated. We submitted Let Loose and the two most recognized alternatives to our manufacturer's laboratory and ran iodometric titration β the standard analytical method for quantifying active oxygen content in a dosed supplement.
Here's what came back:
Regular Magnesium Oxide
0
ppm
No active oxygen
Leading Competitor
2,050
ppm
Baseline ozonated magnesium
Let Loose Daily Capsules
28,186
ppm
13Γ more than competitor
26Γ more than Mg Oxide
26Γ more than Mg Oxide
In plain English: the magnesium does the physical work β pulling water into the colon to soften stool. The active oxygen does the supporting work β helping maintain a healthy gut environment. More oxygen per dose means more of that support in every capsule.
What ozonated magnesium oxide actually is
Ozonation is a process that binds oxygen (Oβ) to another molecule, stabilizing it so it can travel somewhere oxygen doesn't usually reach. Our manufacturer pressurizes medical-grade ozone into magnesium oxide, producing a stable magnesium peroxide complex. Once the capsule dissolves, that complex begins releasing active oxygen into the digestive tract alongside the magnesium itself.
What happens when you take a Let Loose capsule:
1
Water moves in.
Magnesium oxide is an osmotic β it pulls water across the intestinal wall and into the colon. That hydrates stool, softens anything that's been sitting too long, and makes it easier to pass. This is the exact mechanism studied in randomized controlled trials of magnesium oxide for occasional constipation.1
2
Oxygen gets released.
As the magnesium peroxide complex dissociates in the digestive tract, it releases active oxygen into the gut environment β a region of the body where free oxygen is normally very scarce.
The real superpower isn't the magnesium. It's the oxygen.
Most of the constipation category talks about magnesium. Almost no one talks about what oxygen is doing in your gut. Here's what the peer-reviewed research shows.
01
Oxygen helps support a balanced gut environment.
Dysbiosis β an imbalance of gut bacteria often behind bloating and sluggish digestion β is driven in part by the overgrowth of anaerobic species that thrive in a low-oxygen, stagnant colon. In a 2025 peer-reviewed study published in the Journal of Medical Microbiology, ozonated water administration in a dysbiosis model increased beneficial microbiota populations and supported intestinal barrier function, working through the SIRT1βNrf2/HO-1 signaling pathway.3
Plain English: oxygen in the gut helps tilt the balance back toward the bacteria you want.02
Oxygen supports the gut barrier.
The gut barrier is a single-cell-thick layer held together by "tight junction" proteins. When it's doing its job, it lets nutrients through and keeps everything else out. Preclinical research shows that ozone/oxygen therapy supports tight-junction integrity: in one 2025 study, it helped preserve barrier function by activating the aryl hydrocarbon receptor and modulating tryptophan metabolism.4 A separate preclinical model found that medical ozone supported the body's endogenous antioxidant defenses in intestinal tissue.5
Plain English: oxygen helps the gut lining stay sealed the way it's supposed to.03
Oxygen creates an environment less hospitable to unwanted microbes.
Ozone's mechanism of action on bacteria is well characterized. It oxidizes microbial cell membranes, disrupts sulfhydryl groups, and damages pathogen DNA.67 The species most sensitive to this pressure are strict anaerobes β exactly the population that tends to overgrow in a compacted, sluggish colon.
Plain English: the microbes that cause the most trouble in a stagnant gut are the ones that hate oxygen the most.04
Oxygen supports the body's natural antioxidant defenses.
Counterintuitive but well-documented: therapeutic ozone/oxygen mixtures have been shown to increase the body's endogenous antioxidant capacity β upregulating the Nrf2 pathway, glutathione, and superoxide dismutase β rather than deplete it.8
Plain English: a small, controlled dose of active oxygen prompts the body to build more of its own defense system, not less.The takeaway: active oxygen isn't just about clearing waste. It's about supporting the environment in your gut so beneficial bacteria can do their job β and keeping it that way.
Magnesium oxide beats placebo. Ours starts there.
We're keeping the existing RCT evidence on this page because it's the strongest human data for the mineral itself. The reframe: this is the baseline β what magnesium oxide does on its own, before we add active oxygen.
In a 2019 randomized, double-blind, placebo-controlled trial published in the Journal of Neurogastroenterology and Motility, magnesium oxide improved overall symptoms in 70.6% of participants with occasional constipation, compared with 25% on placebo.1 A 2020 follow-up RCT confirmed it: magnesium oxide matched the efficacy of senna (a stimulant laxative) at roughly 68% response, with a different, non-stimulant mechanism.9
Now imagine that baseline β plus 28,186 ppm of active oxygen in every dose.
70.6%
improved on magnesium oxide
(2019 RCT)
(2019 RCT)
25%
improved on placebo
(same trial)
(same trial)
~68%
response β matched senna with a
non-stimulant mechanism (2020 RCT)
non-stimulant mechanism (2020 RCT)
One capsule. Two mechanisms. Non-habit-forming.
Let Loose is the only daily capsule we know of that pairs an RCT-studied osmotic mineral with lab-verified active oxygen at 13Γ the nearest competitor. The magnesium helps move things along. The oxygen supports the environment it's moving through. You take it. You wake up lighter.
These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. If you are pregnant, nursing, taking medication, or have a medical condition, consult your healthcare provider before use.
References
- Mori S, et al. (2019). A Randomized Double-blind Placebo-controlled Trial on the Effect of Magnesium Oxide in Patients With Chronic Constipation. J Neurogastroenterol Motil.
- Fontes B, et al. (2012). Effect of low-dose gaseous ozone on pathogenic bacteria. BMC Infectious Diseases.
- Ozone water enema activates SIRT1-Nrf2/HO-1 pathway to ameliorate gut dysbiosis (2025). Journal of Medical Microbiology.
- Ozone controls the metabolism of tryptophan protecting against sepsis-induced intestinal damage by activating aryl hydrocarbon receptor (2025). World Journal of Gastroenterology.
- Guven A, et al. (2009). Medical ozone therapy reduces oxidative stress and intestinal damage in an experimental model of necrotizing enterocolitis in neonatal rats. Journal of Pediatric Surgery.
- Cho M, et al. (2011). Assessing the microbial oxidative stress mechanism of ozone treatment through the responses of Escherichia coli mutants. Journal of Applied Microbiology.
- Epelle EI, et al. (2023). Ozone application in the food industry: mechanisms of action on microorganisms. PMC.
- Bocci V, et al. (2010). Effect of ozone/oxygen mixture on systemic oxidative stress and organic damage. Toxicology and Industrial Health.
- Morishita D, et al. (2020). Senna Versus Magnesium Oxide for the Treatment of Chronic Constipation: A Randomized, Placebo-Controlled Trial. American Journal of Gastroenterology.
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Digestion is the gateway to nutrition
You don't actually get nourished by what you eat. You get nourished by what you digest.
Modern meals are more complex than the digestive system they're landing in. Processed ingredients, dense proteins, refined oils, engineered foods β all of it asks more of your enzymes than nature ever planned for. Koji Digestive Enzymes help your body break food into the small, usable pieces it actually needs. Meals feel lighter. Digestion runs smoother. Your body gets more out of the food you're already eating.
355
mg of functional enzymes per capsule
4Γ
the total enzyme content of typical mass-market blends
Most enzyme supplements are designed to sound powerful. Ours is dosed to actually perform.
Walk down the enzyme aisle and most labels look impressive: "18 enzymes!" "22-enzyme complex!" Flip them over. The total enzyme content is often around 85 mg, spread across a dozen or more ingredients in trace amounts. That's enough to list on a label. It's not always enough to do the work of digesting a real meal.
Here's the difference:
| Typical Mass-Market Blend | Let Loose Koji Digestive Enzymes |
|---|---|
| ~85 mg total blend | 355 mg per capsule |
| Dozens of enzymes listed, each in trace amounts | 16 enzymes dosed at activity levels sized for real meals |
| Often listed by weight only | Disclosed in enzyme activity units (HUT, DU, FIP, etc.) |
| Commodity sourcing across multiple suppliers | Traditional Japanese Koji fermentation, 150+ year process |
Why activity units matter:
An enzyme's weight tells you how much material is in the capsule, but that includes inactive components too. Activity units tell you how much digestive work it can actually perform. They're not the same thing. HUT (proteaseβ breaks down protein), DU and SU (amylase- break down carbohydrates and starches), FIP (lipase- breaks down fat), GALU (lactase- breaks down lactose and dairy sugars), and ALU (alpha-galactosidase- helps break down beans and complex carbs). These are the standard units used to measure real enzyme function. We list every single one on our label.
What happens when you take a Let Loose capsule before a meal
You take it with water a few minutes before eating. The capsule dissolves in your stomach, releasing the Koji enzyme blend into the digestive tract right as food arrives. Each enzyme works on a specific part of the meal.
The three things that happen:
1
Protein gets a head start in the stomach.
Most animal-derived digestive enzymes don't survive stomach acid β they need enteric coating to work. Koji-derived enzymes are different: fungal proteases are naturally acid-stable and remain active under gastric conditions.1 Our formula includes acid-stable proteases that start breaking protein apart before it ever reaches the small intestine, loosening the structure so later enzymes can finish the job.2
2
The full meal gets covered.
Sixteen enzymes work in parallel β proteases on the steak, amylases on the bread, lipases on the oil, lactase on the cheese, cellulases on the vegetables, alpha-galactosidase on the beans. Whatever's on your plate, something in the capsule is working on it.
3
The good stuff becomes available.
Better breakdown means the nutrients locked inside plant cell walls, protein matrices, and fat droplets are released where your gut can actually absorb them. That's the whole point: you don't benefit from what you eat β you benefit from what you digest and absorb.
What's in each capsule:
See full enzyme breakdown
| Enzyme | Activity per capsule | What it breaks down |
|---|---|---|
| Protease I | 20,000 HUT | Proteins β meats, dairy, legumes |
| Protease II | 50,000 HUT | Proteins β broad pH range |
| Protease (Acid-Stable) | 200 SAPU | Starts protein breakdown in the stomach |
| Peptidase DPP-IV | 72 DPPIV | Peptides and gluten-derived fragments |
| Peptidase | 4,000 HUT | Shorter protein chains |
| Alpha Amylase | 12,000 DU | Starches β bread, rice, potato |
| Alpha Galactosidase | 300 GALU | Complex sugars in beans, cruciferous veg |
| Beta Glucanase | 50 BGU | Beta-glucans in oats, barley |
| Invertase | 400 SU | Sucrose |
| Lactase | 400 ALU | Lactose (dairy sugar) |
| Pectinase | 14 ENDO PG | Pectin in fruits and vegetables |
| Cellulase | 2,000 CU | Cellulose β plant cell walls |
| Hemicellulase | 2,000 HCU | Hemicellulose β plant fiber |
| Xylanase | 300 XU | Xylan β grain fiber |
| Lipase I | 100 FIP | Fats β short/medium-chain |
| Lipase II | 1,300 FIP | Fats β broad-chain |
Activity units: HUT = protein-breaking units. DU = starch-breaking units. FIP = fat-breaking units. GALU, SU, ALU, BGU, CU, HCU, XU, ENDO PG, DPPIV, SAPU = specialist units for specific substrates. Industry standard measurements.
What one capsule is sized for (theoretical)
Based on the activity levels above, one capsule is formulated to support digestion of roughly 40β60 g of protein, 40β80 g of carbohydrate, and 20β25 g of fat β about the size of a steak dinner with a baked potato. These estimates are biochemical modeling, not clinical claims; actual digestion varies by person.
Why we make this with a 1,100-year-old fermentation method
Most enzyme supplements on the market are made by isolating one or two enzymes from fungal or bacterial fermentation, then heavily processing and purifying them into a powder before blending them together. It's efficient. It's cheap. But it's also a narrow, reductionist approach to a very complex problemβbecause real food isn't made of isolated molecules, it's made of complex structures.
Koji (Aspergillus oryzae) is different. It's been cultivated in Japan for over a millennium β originally for miso, soy sauce, and sake β and was domesticated specifically for its enzyme production. A single Koji culture naturally secretes dozens of hydrolytic enzymes in parallel, including 65 endopeptidase and 69 exopeptidase genes' worth of protein-cutting machinery.3 It's the only fungus in the world with a dedicated status as a national fungus of Japan.
1
Koji is built for broad-spectrum digestion.
A single Koji fermentation produces a natural ecosystem of enzymes β proteases, amylases, lipases, cellulases, hemicellulases, xylanases, pectinases, peptidases, esterases β all at once. Because they evolved together, they function together. This is closer to how digestion actually works in the gut (coordinated, overlapping, redundant) than isolated single-enzyme blends can match.4
Plain English: Koji gives you a whole team, not a lineup of solo players.2
Koji enzymes survive stomach acid.
In in-vitro models of human digestion, fungal enzymes remain active under gastric conditions β the high acidity that destroys animal-derived pancreatic enzymes (which is why pancreatic supplements require enteric coating).1 Our acid-stable proteases start breaking down protein in the stomach phase, before food even reaches the small intestine.
Plain English: they start working the moment you swallow.3
Koji has 150+ years of modern medical use.
Koji-derived enzymes aren't new. In 1894, Japanese chemist Jokichi Takamine patented Taka-diastase β a Koji-derived digestive enzyme preparation β and it became one of the first enzyme supplements ever commercialized for stomach upset and overeating.5 Today, A. oryzae is GRAS-designated by the FDA for specified enzyme preparations.5
Plain English: this isn't a trend ingredient. Koji has been helping people digest meals since before the invention of the automobile.4
Koji produces secondary enzymes that isolated blends miss.
Traditional Koji fermentation naturally generates a long tail of secondary enzymes beyond the headline list β cellulases, hemicellulases, xylanases, pectinases, esterases, and additional peptidases. These secondary enzymes specialize in breaking apart the complex structures that trap nutrients: plant cell walls, protein matrices, connective tissues, cellular membranes.
Plain English: before the main enzymes can do their job, something has to open the food up. Koji does both.The takeaway: Koji fermentation is the reason this formula performs like an integrated digestive system instead of a list of isolated enzymes on a label.
What the research says about digestive enzyme supplementation
The category has become well-studied over the last decade. Here's what peer-reviewed human trials have shown.
Multi-enzyme supplementation reduces post-meal bloating.
A 2024 randomized, double-blind, placebo-controlled crossover study of 25 adults with daily post-meal bloating found that a single dose of a multi-digestive-enzyme supplement reduced abdominal distension by 58% at 30 minutes and 68% at 90 minutes compared to placebo. Eighty percent of participants saw a measurable reduction in distension with the enzyme product.6
58%
at 30 minutes
68%
at 90 minutes
compared to placebo
compared to placebo
80%
of participants saw a
measurable reduction
measurable reduction
Enzyme supplements improve digestive comfort over time.
A 2023 randomized, double-blind, placebo-controlled trial of 120 adults with digestive symptoms used a multi-enzyme supplement twice daily for two months. The enzyme group reported improvements in overall quality of life, sleep quality, and a decrease in the severity of post-meal discomfort β with no side effects reported.7 A separate RCT in functional digestive complaints likewise showed statistically significant improvements in bloating, fullness, and post-meal distress versus placebo.8
The underlying mechanism is now well-characterized.
A 2022 study using the INFOGEST in-vitro model of human digestion demonstrated that fungal-derived digestive enzymes meaningfully improve macronutrient hydrolysis β particularly for protein, starch, and fat β under simulated gastric and small intestinal conditions.1 In other words, the mechanism isn't speculative. You can watch it happen in the lab.
Our formula is built on the same class of enzymes these studies used β Koji-fermented, broad-spectrum, dosed by activity unit β at 355 mg per capsule.
One capsule before your two biggest meals. That's the whole ritual.
Take one Let Loose Koji Digestive Enzymes capsule before your two largest meals of the day. For heavier meals, take two. Small habit, meaningful support. And because Koji enzymes are naturally acid-stable, you don't have to time it perfectly β they start working as soon as the capsule dissolves.
Eat what you want. Just digest it better.
These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. If you are pregnant, nursing, taking medication, or have a medical condition, consult your healthcare provider before use. Discontinue if adverse reactions occur.
References
- Morell P, et al. (2022). Fungal digestive enzymes promote macronutrient hydrolysis in the INFOGEST static in vitro simulation of digestion. Food Chemistry.
- Enzyme Technical Association. Orally Administered Enzyme Food Supplement Safety Overview.
- Yang L, et al. (2022). Phenotypic, Genomic, and Transcriptomic Comparison of Industrial Aspergillus oryzae: Analysis of Key Proteolytic Enzymes Produced by Koji Molds. Microbiology Spectrum.
- Daba GM, et al. (2021). The ancient koji mold (Aspergillus oryzae) as a modern biotechnological tool. Bioresources and Bioprocessing.
- Hiroi M, Nagao S. (2021). Medical Application of Substances Derived from Non-Pathogenic Fungi Aspergillus oryzae and A. luchuensis-Containing Koji. Journal of Fungi (PMC).
- Spagnuolo R, et al. (2024). A Multi-Digestive Enzyme and Herbal Dietary Supplement Reduces Bloating in a Single Use in Healthy Adults: A Randomized, Placebo-Controlled, Cross Over Study. Nutrition and Dietary Supplements.
- Graziani C, et al. (2023). Efficacy of digestive enzyme supplementation in functional dyspepsia: A monocentric, randomized, double-blind, placebo-controlled, clinical trial. Biomedicine & Pharmacotherapy.
- Majeed M, et al. (2018). Evaluation of the Safety and Efficacy of a Multienzyme Complex in Patients with Functional Dyspepsia: A Randomized, Double-Blind, Placebo-Controlled Study. Journal of Medicinal Food.
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Your gut is home to trillions of microbes and a delicate barrier that lines your intestines.
When this ecosystem is out of balance, digestion may suffer - manifesting as irregularity, bloating, gas, or slow transit.
TrioBiotic supports gut health with a three-pronged approach:
1
Probiotics
beneficial live microbes that survive digestion and reach the gut.
2
Postbiotics
non-live microbial components that still trigger beneficial responses.
3
Prebiotics & Resistant Starch
fibers that selectively feed good microbes and promote beneficial by-products (short-chain fatty acids) that nourish your gut lining and beyond.
Together, these ingredients promote:
- A stronger gut barrier (meaning fewer leaks & less inflammation)
- A healthier microbiome (more good bacteria, fewer "bad" ones)
- Better digestive regularity and comfort
- Support for metabolic health, immunity, and nutrient absorption
The science-backed ingredients in TrioBiotic
1
Bacillus coagulans (2 Billion CFU)
Mechanism highlights:- Spore-forming resilience β survives stomach acid and bile to reach intestines. ScienceDirect+1
- Produces digestive enzymes and short-chain fatty acids β supports digestion and gut lining health. BioMed Central
- Modulates immune function and microbiota balance. ScienceDirect+1
- Improves IBS symptoms: reduced abdominal pain & increased complete spontaneous bowel movements. Nature
- Safe at 2 Γ 10βΉ CFU/day in adults with diarrhea-predominant IBS. BioMed Central
- Better stool regularity + improved stool consistency
- Reduction in occasional bloating and gas
- Enhanced nutrient absorption and digestive efficiency
- Support for immune and gut-brain axis pathways
2
Bacillus subtilis (2 Billion CFU)
Mechanism highlights:- Spore-forming bacterium supporting a diverse microbiome and producing antimicrobial compounds inhibiting pathogens. Lippincott Journals
- Supports intestinal barrier integrity and healthy immune signalling.
- Helps rebalance gut flora and crowd out less beneficial bacteria
- Strengthens gut lining to reduce "gut leakiness"
- May reduce occasional digestive inflammation, gas, & discomfort
- Supports immune resilience from the gut
3
Akkermansia muciniphila (Heat-Killed AH-39)
Mechanism highlights:- Resides in the mucous layer of the gut, interacting with intestinal lining and influencing metabolism. [Gut+1]
- Even pasteurized (heat-killed) form shows metabolic and barrier benefits. [Nature+1]
- In overweight/insulin-resistant humans, supplementation correlated with improvements in insulin sensitivity, fat mass, and inflammation markers. [Nature+1]
- Strengthens gut barrier & reduces intestinal permeability
- Supports healthy metabolism (weight, insulin, lipid balance)
- Promotes microbiome diversity and long-term digestive resilience
4
Xylooligosaccharides (XOS)
Mechanism highlights:- Specific prebiotic fiber that selectively increases beneficial bacteria like Bifidobacteria. [PMC+1]
- Supports production of short-chain fatty acids and reduces gut pH β beneficial environment for gut health. [Cambridge University Press & Assessment]
- Improves stool frequency & consistency (including relief from functional constipation)
- Supports smoother digestion & reduction in occasional bloating
- Helps maintain a resilient gut environment that supports metabolism and immunity
5
Galactooligosaccharides (GOS)
Mechanism highlights:- Prebiotic fiber broadly supporting Bifidobacteria and Lactobacilli, especially in older adults. [Cambridge University Press & Assessment]
- Associated with improved mineral absorption and immune signalling.
- Supports daily regularity and softer, more comfortable bowel movements
- Nourishes good bacteria and supports overall gut microbiome balance
- Helps support mineral absorption (calcium, magnesium) and immune health
6
Green Banana Powder (Resistant Starch)
Mechanism highlights:- High in resistant starch (RS) which reaches the colon to fuel beneficial microbes and produce short-chain fatty acids (especially butyrate). [PMC+1]
- Supports gut barrier restoration and microbial balance. [PMC+1]
- Promotes stool form, regularity, and digestive comfort
- Supports blood sugar balance and healthy metabolism
- Helps soothe and strengthen the intestinal lining
- Adds a natural prebiotic fiber that supports your microbiome's long-term health
Summary
TrioBiotic from Let Loose is built on multiple layers of science:
- Two robust spore-forming probiotics (B. coagulans + B. subtilis) that survive the digestive tract and reach your intestines alive.
- A next-generation postbiotic (A. muciniphila heat-killed) with demonstrated benefits for gut lining & metabolism.
- Two potent prebiotic fibers (XOS + GOS) and a resistant starch source (green banana) to feed and empower your microbiome.
Together, this formula helps deliver smoother digestion, better regularity, a stronger gut barrier, a more resilient microbiome, and supportive metabolic & immune benefits β tailored for the young, active, health-conscious consumer.
These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. Individual results may vary. Please consult your healthcare provider before beginning any new supplement regimen, especially if you are pregnant, nursing, taking medications, or have a medical condition.
Read the Papers
- Depommier C, et al. Supplementation with Akkermansia muciniphila in overweight/obese insulin-resistant volunteers: a randomized, double-blind, placebo-controlled pilot study. Nature Medicine 2019. [Nature]
- Madempudi R.S., et al. The effect of Bacillus coagulans Unique IS2 in adults with IBS: randomized double-blind study. Scientific Reports 2019. [Nature]
- Yan J., et al. Akkermansia muciniphila: is it the Holy Grail for ameliorating metabolic disorders? Gut Microbes 2021. [PMC]
- Yi W., et al. Xylo-oligosaccharides improve functional constipation by modulating the gut microbiota. Frontiers in Nutrition 2023. [PMC]
- Baek G.H., et al. Prebiotic potential of green banana flour: impact on gut microbiota composition in mice. Frontiers in Nutrition 2023. [Frontiers]
- Walton G.E., et al. A randomised crossover study investigating the effects of galacto-oligosaccharides on the faecal microbiota in men and women over 50 years of age. British Journal of Nutrition 2012. [Cambridge University Press & Assessment]